Protein Labeling Reagents, THERMO FISHER

供应商: THERMO FISHER BRAND
TFBRC10102EA 0 CNY
TFBRC10102
Protein Labeling Reagents, THERMO FISHER
蛋白质提取系统 蛋白提取试剂
Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis.

  • Less hazardous
  • AHA product type
  • Biotin-based, fluorescent detection method
  • Metabolic labeling
  • Proteins labeling target

Detection utilises the chemoselective ligation or 'click' reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® protein analysis detection kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesised proteins together with total glycoproteins, total phosphoproteins or total protein.

Caution: For research use only. Not for use in diagnostic procedures.
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