GoTaq® DNA polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases superior to that of standard Taq polymerase.
The 5X Green and 5X Colourless Reaction Buffers contain a compound that increases sample density, so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye co-migrates with 3 to 5 kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50 bp). The Green Reaction Buffer is recommended for direct-to-gel analysis after amplification and the Colourless Reaction Buffer for post-amplification analysis by fluorescence or absorbance without prior DNA purification.
PCR enhancers, such as betaine and DMSO, have no effect on the colour or characteristics of the GoTaq® Green Reaction Buffer.
The 5X GoTaq® Green and Colourless reaction buffers supplied with GoTaq® DNA Polymerase contain MgCl₂ at a concentration of 7,5 mM for a final concentration of 1,5 mM in the 1X reaction.
Delivery information: Kit includes GoTaq® DNA Polymerase (5 U/µl), 5X Green GoTaq® Buffer, 5X Colourless GoTaq® Buffer.
