Wheat germ extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors).
- Contains tRNA, ribosomes, initiation, elongation and termination factors
- Extract is cleared of endogenous amino acids, mRNA and plant pigments, minimising background translation
The extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine to stimulate the efficiency of chain elongation, magnesium acetate and potassium acetate. Potassium Acetate enhances translation for a wide range of mRNAs. Three amino acid mixtures are provided, which enable use of different radioisotopes.
Delivery information: Includes wheat germ extract, potassium acetate, amino acid mixtures (one each minus leucine, cysteine or methionine), luciferase control RNA.