Library Preparation
sparQ DNA Library Prep kit
sparQ DNA Library Prep Kit is an optimised kit for rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on IlluminaR NGS platforms, and with input amounts from 250 pg - 1 µg.
The streamlined workflow can be completed in under 3 hours with minimal hands-on time. A single tube is used for DNA polishing, ligation, and cleanup. A second tube is used for workflows requiring PCR amplification and a final tube receives the sequencing-ready library.
Technical Literature
Product Flyer - sparQ DNA Library Prep Kit
sparQ DNA Library Prep Kit contains:
- DNA Polishing Enzyme Mix
- DNA Polishing Buffer
- DNA Ligase
- DNA Rapid Ligation Buffer
- HiFi PCR Master Mix (2X)
- Primer Mix
SparQ Adapter Barcode Sets
sparQ Adapter Barcode Sets A and B are single index barcoded oligonucleotide adapters designed for use with the Quantabio sparQ DNA Library Prep Kits in the construction of libraries for Illumina NGS platforms. Each sparQ Adapter Barcode Set contains 12 single index barcodes for 96 rxns.
| sparQ Adapter Barcode Set A | sparQ Adapter Barcode Set B | ||
| Barcode Number | Barcode Sequence | Barcode Number | Barcode Sequence |
| 2 | CGATGT | 1 | ATCACG |
| 4 | TGACCA | 3 | TTAGGC |
| 5 | ACAGTG | 8 | ACTTGA |
| 6 | GCCAAT | 9 | GATCAG |
| 7 | CAGATC | 10 | TAGCTT |
| 12 | CTTGTA | 11 | GGCTAC |
| 13 | AGTCAA | 20 | GTGGCC |
| 14 | AGTTCC | 21 | GTTTCG |
| 15 | ATGTCA | 22 | CGTACG |
| 16 | CCGTCC | 23 | GAGTGG |
| 18 | GTCCGC | 25 | ACTGAT |
| 19 | GTGAAA | 27 | ATTCCT |