您在搜索: Cloning+Buffers

目录号: (VWRCK656-500ML)

供应商: VWR Chemicals

描述: A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

计量单位: 1 * 500 毫升

SDS 正在促销

供应商: VWR Chemicals

描述: Running buffers for both 1-D and 2-D protein gel electrophoresis.

SDS

供应商: VWR Chemicals

描述: TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

SDS

供应商: VWR Chemicals

描述: Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

SDS

供应商: VWR Chemicals

描述: TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

SDS 证书

目录号: (EBIO14-5963-82)

供应商: EBIOSCIENCE

描述: Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection. Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections. Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

计量单位: 1 * 100 µG

正在促销

供应商: VWR Chemicals

描述: Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

SDS 证书

目录号: (VWRCK855-500ML)

供应商: VWR Chemicals

描述: Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

计量单位: 1 * 500 毫升

SDS 正在促销

供应商: VWR Chemicals

描述: HEPES buffer solution pH 7.3 (1 mol/l), VWR®

SDS 证书

目录号: (VWRCE691-500ML)

供应商: VWR Chemicals

描述: TRIS buffer solution pH 7,5 (1 mol/l), VWR®

计量单位: 1 * 500 毫升

SDS 证书 正在促销

目录号: (VWRCE442-500ML)

供应商: VWR Chemicals

描述: Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

计量单位: 1 * 500 毫升

SDS 证书 正在促销

目录号: (VWRC437117K)

供应商: VWR Chemicals

描述: Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

计量单位: 1 * 10 升

SDS 证书 正在促销

目录号: (VWRCM232-39.8G-5PK)

供应商: VWR Chemicals

描述: Each pack of powdered blocking buffer makes one liter of 1X solution.

计量单位: 1 * 199 克

正在促销

供应商: VWR Chemicals

描述: Sodium acetate buffer, VWR®, 3 M, pH 值: 5,2

SDS 证书

目录号: (VWRCK856-500ML)

供应商: VWR Chemicals

描述: Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

计量单位: 1 * 500 毫升

SDS 证书 正在促销

目录号: (VWRCM334-100ML)

供应商: VWR Chemicals

描述: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

计量单位: 1 * 100 毫升

正在促销