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描述: These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.

目录号: PIER37539

计量单位: 1 * 1 升

供应商: Thermo Fisher Scientific

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描述: Designed specifically for immunoassay applications, this range of products have binding surfaces that include Immulon®, universal binding (UB) and enhanced binding (EB). These make good experimental design easier, allowing development of assays with stringent pH, buffer, pKa and resin requirements.

目录号: NUNC6309

计量单位: 1 * 100 件

供应商: Thermo Fisher Scientific

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描述: The Nunc™ Edge plate has a design that minimises evaporation and the risks associated with the edge effect. When filled with sterile water or media, the surrounding moat acts as an evaporation buffer during extended incubation, yielding consistent, viable cells across the entire 96-well plate that in turn enhances productivity for cell-based assays. Enabling the use of all 96 wells allows researchers to plan experiments more efficiently, resulting in potentially less cost and related product waste.

目录号: NUNC167425

计量单位: 1 * 50 件

供应商: Thermo Fisher Scientific

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描述: 5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

目录号: EBIO25-0251-82

计量单位: 1 * 100 µG

供应商: EBIOSCIENCE

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描述: 5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

目录号: EBIO47-0451-82

计量单位: 1 * 100 µG

供应商: EBIOSCIENCE

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描述: 5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

目录号: EBIO25-0731-82

计量单位: 1 * 100 µG

供应商: EBIOSCIENCE

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描述: Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection. Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections. Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

目录号: EBIO14-5963-82

计量单位: 1 * 100 µG

供应商: EBIOSCIENCE

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描述: These tablets provide a rapid and convenient method of preparing a buffer solution.

目录号: VWRC331552S

计量单位: 1 * 50 Tablet

供应商: VWR Chemicals

SDS 证书 正在促销

描述: Tris (hydroxymethyl) aminomethane.

目录号: VWRCE199-500ML

计量单位: 1 * 500 毫升

供应商: VWR Chemicals

SDS 证书 正在促销

描述: For transfer of protein to membranes for sequencing or blotting.

目录号: VWRCK872-500ML

计量单位: 1 * 500 毫升

供应商: VWR Chemicals

SDS 正在促销

描述: Cloning Buffer, SM BUFFER

目录号: VWRCJ614-500ML

计量单位: 1 * 500 毫升

供应商: VWR Chemicals

证书 正在促销

描述: MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

目录号: VWRCE526-500ML

计量单位: 1 * 500 毫升

供应商: VWR Chemicals

SDS 正在促销

描述: SSC buffer is a hybridisation buffer for Northern and Southern blots.

目录号: VWRC0804-4L

计量单位: 1 * 4 升

供应商: VWR Chemicals

SDS 证书 正在促销

描述: Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

目录号: VWRCJ373-4L

计量单位: 1 * 4 升

供应商: VWR Chemicals

SDS 证书 正在促销

描述: Running buffers for both 1-D and 2-D protein gel electrophoresis.

目录号: VWRC0307-1L

计量单位: 1 * 1 升

供应商: VWR Chemicals

SDS 正在促销

描述: Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

目录号: VWRCE442-500ML

计量单位: 1 * 500 毫升

供应商: VWR Chemicals

SDS 证书 正在促销