BACC Genomic DNA extraction kits, Nucleon™

供应商: Cytiva

RPN8501 RPN8512
GEHERPN8501EA 0 CNY
GEHERPN8501 GEHERPN8512
BACC Genomic DNA extraction kits, Nucleon™
核酸试剂 核酸纯化试剂盒和试剂

Nucleon™ BACC Genomic DNA Extraction Kits were developed for rapid extraction of high quality, high molecular weight genomic DNA from blood and cell cultures.


  • Developed for rapid extraction of high-quality, high molecular weight genomic DNA from whole blood and cell cultures
  • Recovers DNA ranging in size from 23 to 250 kbp as determined by pulse-field gel electrophoresis
  • Features a phenol-free protocol requiring only 30 min to complete


The Nucleon™ proprietary resin is added following cell lysis, deproteinisation with sodium perchlorate, and a single chloroform extraction. Aspiration of the top layer of nucleic acids is simplified and volume of usable sample maximized by a barrier formed between the two layers.


Parameter
Application: Rapid extraction of high-quality, high molecular weight genomic DNA from cell cultures and blood.
Protocol length: 30 min
Sample type: Cell cultures and blood
Yield: 370-440 µg DNA/mg tissue
Mean Purity (A 260/280): 1.8


Nucleon™ BACC1: For 50 preps of 1 ml of whole blood or cultured cells (1 to 3×10⁶ cells/ml)


Nucleon™ BACC3: For 50 preps of 10 ml of whole blood (kit contains all reagents necessary for 50×10 ml blood extractions).

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