DL-二硫苏糖醇 99,4 Biotechnology
供应商: VWR Chemicals
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Warning
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Synonyms:
1,4-二巯基苏糖醇, 克莱兰氏试剂, DTT
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
分子式:
C₄H₁₀O₂S₂ 分子量: 154.25 g/mol 沸点: < 125 °C (1013 hPa) 溶点: 42…44 °C 闪点: >110 °C (open cup) 存储条件: Refrigerator |
MDL编号:
MFCD00004877 CAS号: 3483-12-3 EINECS: 222-468-7 |
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产品技术规格
Abs.@283 nm (0.02 M, Water) | ≤0.05 |
DNase | none detected |
Loss on Drying | <0.5% |
Melting Point | 39 – 43 °C |
Oxidised DTT | <0.5% |
Protease | none detected |
Purity (-SH Content) | >99.4% |
RNase | none detected |
Solubility (5%, Water) | pass |
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