The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.
- Bright NanoLuc® reporter enables use in applications where sensitivity is limited
- Add-and-read simplicity allows scaling from bench to HTS
- Extended bright light output optimised for batch and continuous processing
- Low background formulation offers increased sensitivity
The reagent is prepared by mixing Nano-Glo® Luciferase Assay Substrate and Nano-Glo® Luciferase Assay Buffer. The reagent contains an integral lysis buffer allowing use directly on cells expressing NanoLuc® luciferase or the culture media when luciferase is secreted.