Oxidative stress detection kit, ROS-Glo™ H₂O₂ assay
供应商: Promega
The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.
- Fast assay involves a simple two-reagent-addition protocol
- Substrate reacts directly with H₂O₂, no need for HRP as a coupling enzyme
- Multiplex with a cytotoxicity assay for more data per well
The ROS-Glo H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatized luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. Addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.
The addition of ROS-Glo Detection Solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.
Delivery information: The kit contains H₂O₂ substrate (10 mM), signal enhancer solution, H₂O₂ substrate dilution buffer, d-cysteine (100X), luciferin detection reagent and reconstitution buffer.
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