3,3′,5,5′-四甲基联苯胺 membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component

供应商: VWR Chemicals

Synonyms: TMB

VWRCK830-100MLEA 1405 CNY
VWRCK830-100ML
3,3′,5,5′-四甲基联苯胺 membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component
3,3′,5,5′-四甲基联苯胺

Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.


  • Ready to use solution


Reaction of the substrate with HRP generates a free-radical cation that exhibits blue colour with an absorption maximum at 653 nm. Acidification of the reaction by addition of sulfuric acid yields a yellow-coloured diimine terminal oxidation product, which absorbs light at 450 nm.


TMB Plus liquid component substrate is optimised with TMB and hydrogen peroxide concentrations that allow a linear response to typical HRP concentrations utilised in immunologic assays.


订购须知 Contains 2,08 mmol/l TMB, hydrogen peroxide, citric acid buffer pH 3,3 and additional proprietary, non toxic stabilisers.


Packaging: Packed in plastic bottle.

分子式: C₁₆H₂₀N₂
分子量: 240.35 g/mol
沸点: 402
MDL编号: MFCD00007748
CAS号: 54827-17-7

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产品技术规格

Abs.@450nm (50%, 1M H2SO4) ≤ 0.1
Abs.@650nm (undiluted) ≤ 0.1
Assay (P/F) PASS / FAIL
pH @25C (undiluted) 2.6 - 3.6


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